Route 95 is a highway in southern Missouri. Its northern terminus is at Route 32 at Lynchburg in Laclede County. Its southern terminus is at U.S. Route 160 at Lutie about 2.5 miles west of the Theodosia arm of Bull Shoals Lake in Ozark County.

A short 3.3 miles (5.3 km) section forms a wrong-way concurrency with Route 5; Route 95 is marked as "North Route 95" but goes south at this location.

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The section of Route 95 north of Wasola to Lutie was originally a section of Route 5A, a branch of Route 5.

County	Location	mi	km	Destinations	Notes
Ozark	Lutie	0.000	0.000	US 160 to Route 125 – Theodosia
	Wasola	20.577	33.115	Route 5 north – Ava	Northern end of Route 5 overlap
	Barren Fork Township	23.822	38.338	Route 5 south – Gainesville	Southern end of Route 5 overlap
Douglas	Gentryville	41.523	66.825	Route 14 – Twin Bridges, Ava
	Wood Township	51.022	82.112	Route 76 to Route 5 – Willow Springs
Wright	Mountain Grove	62.931	101.278	US 60 Bus. (Third Street)
		64.070	103.111	US 60 – Willow Springs, Springfield
	Van Buren Township	76.037	122.370	Route 38 – Bendavis, Hartville
Laclede	Lynchburg	93.051	149.751	Route 32 – Plato, Lebanon
1.000 mi = 1.609 km; 1.000 km = 0.621 mi       Concurrency terminus