Markinch railway station is a railway station in Markinch, Fife, Scotland, which serves the Glenrothes, Leslie and Levenmouth areas of Fife.

The station is managed by ScotRail and is on the main Edinburgh to Aberdeen Line, 33+1⁄4 miles (53.5 km) north of Edinburgh Waverley. The station was rebuilt to include a new building and park and ride facilities for passengers travelling to Glenrothes and the East of Fife. There are up to ten buses an hour connecting the station with Glenrothes and four to the Levenmouth area. A cycle route has also been completed along the former Leslie Railway into Glenrothes.

The now-closed Leslie Railway left the mainline immediately south of this station.

Most ScotRail services from Edinburgh to Dundee and Perth (each hourly), and some services to Inverness and Aberdeen call at Markinch. Southbound has two trains per hour to Edinburgh. There is one direct service from Glasgow Queen Street each weekday evening.

Sunday services are somewhat irregular and run every two hours in the morning and early afternoon but more frequently thereafter. They run to and from Aberdeen or Inverness northbound.

As of 21 May 2023, CrossCountry operate one northbound train per day to Aberdeen and one southbound train a day to Penzance on weekdays. On Sundays, the northbound service terminates at Dundee and the southbound train terminates at Plymouth.

Preceding station	National Rail			Following station
Kirkcaldy		ScotRail Edinburgh–Dundee line		Ladybank
Kirkcaldy		ScotRail Fife Circle Line		Terminus
Kirkcaldy		CrossCountry Cross Country Route		Ladybank
	Historical railways
Falkland Road Line open, station closed		North British Railway Edinburgh and Northern Railway		Thornton Junction Line open, station closed

• Train times and station information for Markinch railway station from National Rail